Final California 2018 Integrated Report (303(d) List/305(b) Report)

Supporting Information

Regional Board 9 - San Diego Region

Water Body Name:	Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon
Water Body ID:	CAC9011200020090505110014
Water Body Type:	Coastal & Bay Shoreline


DECISION ID	77516	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

Pollutant:	Indicator Bacteria
Final Listing Decision:	Delist from 303(d) list (TMDL required list)
Last Listing Cycle's Final Listing Decision:	Delist from 303(d) list (TMDL required list)(2016)
Revision Status	Original
Reason for Delisting:	Applicable WQS attained; reason for recovery unspecified
Impairment from Pollutant or Pollution:	Pollutant

Regional Board Conclusion:	This pollutant is being considered for removal from the CWA section 303(d) List under section 4.2 of the Listing Policy. Under this section a single line of evidence is necessary to assess listing status. Nine lines of evidence are available in the administrative record to assess this pollutant. Including the latest data, 11 of the 367 samples exceeded the water quality objective for enterococcus of a geomean of 35/100 ml in a 30-day period for the protection of REC-1. Based on the readily available data and information, the weight of evidence indicates that there is sufficient justification for removing this water segment-pollutant combination from the CWA section 303(d) List. This conclusion is based on the staff findings that: 1. The data used satisfies the data quality requirements of section 6.1.4 of the Policy. 2. The data used satisfies the data quantity requirements of section 6.1.5 of the Policy. 3. Including the latest data, 11 of the 367 samples exceeded the water quality objective for enterococcus of a geomean of 35/100 ml in a 30-day period for the protection of REC-1 and this does not exceed the allowable frequency listed in Table 4.2 of the Listing Policy. 4. Pursuant to section 4.11 of the Listing Policy, no additional data and information are available indicating that standards are not met.

Regional Board Decision Recommendation:	After review of the available data and information, RWQCB staff concludes that the water body-pollutant combination should be removed from the section 303(d) list because applicable water quality standards for the pollutant are not being exceeded.

State Board Review of Regional Board Conclusion and Recommendation:

State Board Decision Recommendation:	After review of this Regional Board decision, SWRCB staff recommend the decision be approved by the State Board.

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

29310

Pollutant:

Enterococcus

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

Number of Exceedances:

Data and Information Type:

PWS pathogen monitoring (ambient water)

Data Used to Assess Water Quality:

Beach monitoring data was collected by the County of Orange from January 1999 through December 2007. A total of 939 single samples. Between March 2002 and December 2007, 51 samples were correlated with storm events. Of the 51 samples, 18 exceeded the single sample water quality objective. This information will not be used in determining a listing decision, but is of interest to the Regional Board and has been included here as additional anecdotal information.

Data Reference:

Ocean Bacteriological Data Evaluation for City of Laguna Beach, 1999 through 2006, Final Report

National Weather Service Forecast Office, San Diego, California, Chronological Regional Temperature and Precipitation Listings by Station

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

Single Sample Maximum: Enterococcus density shall not exceed 104 per 100 ml;

Geomean: Enterococcus density shall not exceed 35 per 100 ml (SWRCB, 2005).

Objective/Criterion Reference:

Water Quality Control Plan Ocean Waters of California. California Ocean Plan 2005. Sacramento, CA: State Water Resources Control Board, California Environmental Protection Agency

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Samples were collected from Bluebird Canyon, station id S15, in the Laguna HSA. Lat/Long: 33.52963/-117.77359.

Temporal Representation:

Samples were collected weekly from January 1999 through December 2007; however, rainfall data is only available from March 2002 through December 2007.

Environmental Conditions:

Comparisons were made for all data and data associated with storm events. A storm event was defined as 0.2 inches of rainfall within a 72 hour period.

Analyzing only storm water bacteria data is important because the majority of beach monitoring data covers the months of April through October. The data may not capture the full impacts of seasonal storm events and high bacteria levels due to storm water runoff because much of the wet season for Southern California occurs in November through March.

QAPP Information:

Quality control for the bacteria analysis portion of this study was conducted in accordance with the County of Orange Quality Assessment/Quality Control document.

QAPP Information Reference(s):

County of Orange. Quality Assurance/Quality Control Manual, February 2004 Bacteria specific

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

31081

Pollutant:

Enterococcus

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

Number of Exceedances:

Data and Information Type:

PWS pathogen monitoring (ambient water)

Data Used to Assess Water Quality:

Orange County collected Beach monitoring data within the time period from January 1999 through December 2006.
A total of 56 monthly geomeans were calculated for data collected during the time frame of April 1st to October 31st (AB411 data) within the aforementioned time period. Of the 56 geomeans zero exceeded the geomean water quality objective.

Data Reference:

Ocean Bacteriological Data Evaluation for City of Laguna Beach, 1999 through 2006, Final Report

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

Geomean: Enterococcus density shall not exceed 35 per 100 ml within any 30 day period (SWRCB, 2005).

Objective/Criterion Reference:

Water Quality Control Plan Ocean Waters of California. California Ocean Plan 2005. Sacramento, CA: State Water Resources Control Board, California Environmental Protection Agency

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Samples were collected from Bluebird Canyon, station id S15, in the Laguna HSA. Lat/Long: 33.52963/-117.77359.

Temporal Representation:

Samples were collected at least once a week within the time period from January 1999 through December 2006.
The data assessed is AB411 data which is data collected during the time frame of April 1st to October 31st (summer months) within the aforementioned time period.

Environmental Conditions:

QAPP Information:

Quality control for the bacteria analysis portion of this study was conducted in accordance with the County of Orange Quality Assessment/Quality Control document.

QAPP Information Reference(s):

County of Orange. Quality Assurance/Quality Control Manual, February 2004 Bacteria specific

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

74882

Pollutant:

Enterococcus

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

121

Number of Exceedances:

Data and Information Type:

Not Specified

Data Used to Assess Water Quality:

Zero of the 121 geomeans exceeded the objective.

Data Reference:

Data for Region 9 Beach Watch.

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

The geometric mean standard for entercoccus states that the entercoccus density shall not exceed 35 per 100 mL. Water Quality Control Plan for Ocean Waters 2009.

Objective/Criterion Reference:

California Ocean Plan Water Quality Control Plan Ocean Waters of California 2009

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Samples were collected at the Bluebird Canyon site.

Temporal Representation:

Samples were collected from January 2008 to August 2010.

Environmental Conditions:

QAPP Information:

The samples were collected for the Beach Watch program.

QAPP Information Reference(s):

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

29296

Pollutant:

Enterococcus

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

108

Number of Exceedances:

Data and Information Type:

PWS pathogen monitoring (ambient water)

Data Used to Assess Water Quality:

Beach monitoring data was collected by the County of Orange from January 1999 through December 2007. A total of 939 single samples were collected with 108 monthly geomeans calculated. Of the 108 geomeans, 11 exceeded the geomean water quality objective.

Data Reference:

Ocean Bacteriological Data Evaluation for City of Laguna Beach, 1999 through 2006, Final Report

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

Single Sample Maximum: Enterococcus density shall not exceed 104 per 100 ml;

Geomean: Enterococcus density shall not exceed 35 per 100 ml (SWRCB, 2005).

Objective/Criterion Reference:

Water Quality Control Plan Ocean Waters of California. California Ocean Plan 2005. Sacramento, CA: State Water Resources Control Board, California Environmental Protection Agency

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Samples were collected from Bluebird Canyon, station id S15, in the Laguna HSA. Lat/Long: 33.52963/-117.77359.

Temporal Representation:

Samples were collected weekly from January 1999 through December 2007; however, rainfall data is only available from March 2002 through December 2007.

Environmental Conditions:

QAPP Information:

Quality control for the bacteria analysis portion of this study was conducted in accordance with the County of Orange Quality Assessment/Quality Control document.

QAPP Information Reference(s):

County of Orange. Quality Assurance/Quality Control Manual, February 2004 Bacteria specific

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

74881

Pollutant:

Enterococcus

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

138

Number of Exceedances:

Data and Information Type:

Not Specified

Data Used to Assess Water Quality:

None of the 138 geomeans exceeded the enterococcus objective. For this station, samples were collected from surfzone upcoast and surfzone downcoast. The higher of the two values was used for the geomean calculation.

Data Reference:

Data for Various Waterbodies in Region 8 and Region 9, 2006-2009.

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

The geometric mean for enterococcus shall not exceed 35 MPN/100 mL. Water Quality Control Plan for the San Diego Basin. California Ocean Plan.

Objective/Criterion Reference:

Water Quality Control Plan for the San Diego Basin

Objective/Criterion Reference:

California Ocean Plan Water Quality Control Plan Ocean Waters of California 2009

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

The samples were collected from Coastal Stormdrain at Bluebird Canyon Drive (surfzone upcoast and surfzone downcoast).

Temporal Representation:

The samples were collected once a week from July 2006 to 2009.

Environmental Conditions:

QAPP Information:

The samples were collected and analyzed under the Quality Assurance Management Plan for The Orange County Stormwater Program. Coastal Stormdrain Outfall Monitoring Program.

QAPP Information Reference(s):

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

74880

Pollutant:

Enterococcus

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

149

Number of Exceedances:

Data and Information Type:

Not Specified

Data Used to Assess Water Quality:

Zero of the 149 samples exceeded the enterococcus objective. For this station, samples were collected from surfzone upcoast and surfzone downcoast. The results represent the higher of the two values.

Data Reference:

Data for Various Waterbodies in Region 8 and Region 9, 2006-2009.

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

The single sample enterococcus concentration shall not exceed more than 104/100ml. Water Quality Control Plan for Ocean Waters of California. Region 9 Basion Plan.

Objective/Criterion Reference:

Water Quality Control Plan for the San Diego Basin

Objective/Criterion Reference:

California Ocean Plan Water Quality Control Plan Ocean Waters of California 2009

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

The samples were collected from site BLUBRD, Coastal Stormdrain at Bluebird Canyon Drive (surzone upcoast and downcoast).

Temporal Representation:

The samples were collected once a week from July 2006 to April 2009.

Environmental Conditions:

QAPP Information:

The samples were collected and analyzed under the Quality Assurance Management Plan for The Orange County Stormwater Program. Coastal Stormdrain Outfall Monitoring Program.

QAPP Information Reference(s):

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

29309

Pollutant:

Enterococcus

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

939

Number of Exceedances:

108

Data and Information Type:

PWS pathogen monitoring (ambient water)

Data Used to Assess Water Quality:

Beach monitoring data was collected by the County of Orange from January 1999 through December 2007. A total of 939 single samples were collected with 108 exceeding the single sample water quality objective.

Data Reference:

Ocean Bacteriological Data Evaluation for City of Laguna Beach, 1999 through 2006, Final Report

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

Single Sample Maximum: Enterococcus density shall not exceed 104 per 100 ml;

Geomean: Enterococcus density shall not exceed 35 per 100 ml (SWRCB, 2005).

Objective/Criterion Reference:

Water Quality Control Plan Ocean Waters of California. California Ocean Plan 2005. Sacramento, CA: State Water Resources Control Board, California Environmental Protection Agency

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Samples were collected from Bluebird Canyon, station id S15, in the Laguna HSA. Lat/Long: 33.52963/-117.77359.

Temporal Representation:

Samples were collected weekly from January 1999 through December 2007; however, rainfall data is only available from March 2002 through December 2007.

Environmental Conditions:

QAPP Information:

Quality control for the bacteria analysis portion of this study was conducted in accordance with the County of Orange Quality Assessment/Quality Control document.

QAPP Information Reference(s):

County of Orange. Quality Assurance/Quality Control Manual, February 2004 Bacteria specific

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

29316

Pollutant:

Fecal Coliform

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

Number of Exceedances:

Data and Information Type:

PWS pathogen monitoring (ambient water)

Data Used to Assess Water Quality:

Beach monitoring data was collected by the County of Orange from January 1999 through December 2007. A total of 939 single samples were collected. From March 2002 through December 2007, 50 samples were correlated with storm evernts. Of the 50 samples, six exceeded the single sample water quality objective. This information will not be used in determining a listing decision, but is of interest to the Regional Board and has been included here as additional anecdotal information.

Data Reference:

Ocean Bacteriological Data Evaluation for City of Laguna Beach, 1999 through 2006, Final Report

National Weather Service Forecast Office, San Diego, California, Chronological Regional Temperature and Precipitation Listings by Station

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

Single Sample Maximum: Fecal coliform density shall not exceed 400 per 100 ml;

Geomean: Fecal coliform density shall not exceed 200 per 100ml (SWRCB, 2005).

Objective/Criterion Reference:

Water Quality Control Plan Ocean Waters of California. California Ocean Plan 2005. Sacramento, CA: State Water Resources Control Board, California Environmental Protection Agency

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Samples were collected from Bluebird Canyon, station id S15, in the Laguna HSA. Lat/Long: 33.52963/-117.77359.

Temporal Representation:

Samples were collected weekly from January 1999 through December 2007; however, rainfall data is only available from March 2002 through December 2007.

Environmental Conditions:

QAPP Information:

Quality control for the bacteria analysis portion of this study was conducted in accordance with the County of Orange Quality Assessment/Quality Control document.

QAPP Information Reference(s):

County of Orange. Quality Assurance/Quality Control Manual, February 2004 Bacteria specific

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

31080

Pollutant:

Fecal Coliform

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

Number of Exceedances:

Data and Information Type:

PWS pathogen monitoring (ambient water)

Data Used to Assess Water Quality:

Data Reference:

Ocean Bacteriological Data Evaluation for City of Laguna Beach, 1999 through 2006, Final Report

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

Geomean: Fecal coliform density shall not exceed 200 per 100ml within any 30 day period (SWRCB, 2005).

Objective/Criterion Reference:

Water Quality Control Plan Ocean Waters of California. California Ocean Plan 2005. Sacramento, CA: State Water Resources Control Board, California Environmental Protection Agency

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Samples were collected from Bluebird Canyon, station id S15, in the Laguna HSA. Lat/Long: 33.52963/-117.77359.

Temporal Representation:

Environmental Conditions:

QAPP Information:

Quality control for the bacteria analysis portion of this study was conducted in accordance with the County of Orange Quality Assessment/Quality Control document.

QAPP Information Reference(s):

County of Orange. Quality Assurance/Quality Control Manual, February 2004 Bacteria specific

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

74883

Pollutant:

Fecal Coliform

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

138

Number of Exceedances:

Data and Information Type:

Not Specified

Data Used to Assess Water Quality:

None of the 138 geomeans exceeded the fecal coliform objective. For this station, samples were collected from surfzone upcoast and surfzone downcoast. The higher of the two values was used for the geomean calculation.

Data Reference:

Data for Various Waterbodies in Region 8 and Region 9, 2006-2009.

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

The geometric mean for fecal coliform shall not exceed more than 200/100ml. Water Quality Control Plan for the San Diego Basin. California Ocean Plan.

Objective/Criterion Reference:

Water Quality Control Plan for the San Diego Basin

Objective/Criterion Reference:

California Ocean Plan Water Quality Control Plan Ocean Waters of California 2009

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

The samples were collected from Coastal Coastal Stormdrain at Bluebird Canyon Drive (surfzone upcoast and surfzone downcoast).

Temporal Representation:

The samples were collected once a week from July 2006 to 2009.

Environmental Conditions:

QAPP Information:

The samples were collected and analyzed under the Quality Assurance Management Plan for The Orange County Stormwater Program. Coastal Stormdrain Outfall Monitoring Program.

QAPP Information Reference(s):

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

74901

Pollutant:

Fecal Coliform

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

149

Number of Exceedances:

Data and Information Type:

Not Specified

Data Used to Assess Water Quality:

Zero of the 149 samples exceeded the fecal coliform objective. For this station, samples were collected from surfzone upcoast and surfzone downcoast. The results represent the higher of the two values.

Data Reference:

Data for Various Waterbodies in Region 8 and Region 9, 2006-2009.

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

The single sample fecal coliform concentration shall not exceed more than 400/100ml. Water Quality Control Plan for Ocean Waters of California. Region 9 Basion Plan.

Objective/Criterion Reference:

Water Quality Control Plan for the San Diego Basin

Objective/Criterion Reference:

California Ocean Plan Water Quality Control Plan Ocean Waters of California 2009

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

The samples were collected from site BLUBRD, Coastal Stormdrain at Bluebird Canyon Drive (surfzone upcoast and downcoast).

Temporal Representation:

The samples were collected once a week from July 2006 to June 2009.

Environmental Conditions:

QAPP Information:

The samples were collected and analyzed under the Quality Assurance Management Plan for The Orange County Stormwater Program. Coastal Stormdrain Outfall Monitoring Program.

QAPP Information Reference(s):

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

29315

Pollutant:

Fecal Coliform

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

108

Number of Exceedances:

Data and Information Type:

PWS pathogen monitoring (ambient water)

Data Used to Assess Water Quality:

Beach monitoring data was collected by the County of Orange from January 1999 through December 2007. A total of 939 single samples were collected with 108 monthly goemeans calculated. Of the 108 geomeans, none exceeded the geomean water quality objective.

Data Reference:

Ocean Bacteriological Data Evaluation for City of Laguna Beach, 1999 through 2006, Final Report

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

Single Sample Maximum: Fecal coliform density shall not exceed 400 per 100 ml;

Geomean: Fecal coliform density shall not exceed 200 per 100ml (SWRCB, 2005).

Objective/Criterion Reference:

Water Quality Control Plan Ocean Waters of California. California Ocean Plan 2005. Sacramento, CA: State Water Resources Control Board, California Environmental Protection Agency

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Samples were collected from Bluebird Canyon, station id S15, in the Laguna HSA. Lat/Long: 33.52963/-117.77359.

Temporal Representation:

Samples were collected weekly from January 1999 through December 2007; however, rainfall data is only available from March 2002 through December 2007.

Environmental Conditions:

QAPP Information:

Quality control for the bacteria analysis portion of this study was conducted in accordance with the County of Orange Quality Assessment/Quality Control document.

QAPP Information Reference(s):

County of Orange. Quality Assurance/Quality Control Manual, February 2004 Bacteria specific

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

29314

Pollutant:

Fecal Coliform

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

939

Number of Exceedances:

Data and Information Type:

PWS pathogen monitoring (ambient water)

Data Used to Assess Water Quality:

Beach monitoring data was collected by the County of Orange from January 1999 through December 2007. A total of 939 single samples were collected with 15 samples exceeding the single sample water quality objective.

Data Reference:

Ocean Bacteriological Data Evaluation for City of Laguna Beach, 1999 through 2006, Final Report

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

Single Sample Maximum: Fecal coliform density shall not exceed 400 per 100 ml;

Geomean: Fecal coliform density shall not exceed 200 per 100ml (SWRCB, 2005).

Objective/Criterion Reference:

Water Quality Control Plan Ocean Waters of California. California Ocean Plan 2005. Sacramento, CA: State Water Resources Control Board, California Environmental Protection Agency

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Samples were collected from Bluebird Canyon, station id S15, in the Laguna HSA. Lat/Long: 33.52963/-117.77359.

Temporal Representation:

Samples were collected weekly from January 1999 through December 2007; however, rainfall data is only available from March 2002 through December 2007.

Environmental Conditions:

QAPP Information:

Quality control for the bacteria analysis portion of this study was conducted in accordance with the County of Orange Quality Assessment/Quality Control document.

QAPP Information Reference(s):

County of Orange. Quality Assurance/Quality Control Manual, February 2004 Bacteria specific

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

74902

Pollutant:

Fecal Coliform

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

121

Number of Exceedances:

Data and Information Type:

Not Specified

Data Used to Assess Water Quality:

Zero of the 121 geomeans exceeded the objective.

Data Reference:

Data for Region 9 Beach Watch.

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

The standard for fecal coliform states that the coliform density shall not exceed 200 per 100 mL. Water Quality Control Plan for Ocean Waters 2009.

Objective/Criterion Reference:

California Ocean Plan Water Quality Control Plan Ocean Waters of California 2009

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Samples were collected at the Bluebird Canyon site.

Temporal Representation:

Samples were collected from January 2008 to August 2010.

Environmental Conditions:

QAPP Information:

The samples were collected for the beach watch program.

QAPP Information Reference(s):

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

29325

Pollutant:

Indicator Bacteria

LOE Subgroup:

Health Advisories

Matrix:

-N/A

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

2555

Number of Exceedances:

204

Data and Information Type:

PWS pathogen monitoring (ambient water)

Data Used to Assess Water Quality:

For the period from January 2000 to December 2006, there were and 204 beach postings days for this section of shoreline. Bacteriological samples are collected on a weekly basis at approximately 150 ocean and bay location in Orange County. When a bacteriological sample exceeds water quality standards, signs are posted indicating that waters have exceeded public health standards.

Data and information on the number of beach posting were summarized by the County of Orange in their Annual Ocean and Bay Water Quality Report, March 2007. The reporting period was from January 2000 -December 2006. Data used was the number of days the beach was posting when the ocean or bay failed to meet the bacteriological standards.

Data Reference:

County of Orange. March 2007. Annual Ocean and Bay Water Quality Report, 2006

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

California Code of Regulations. Title 17, Section 7960.

(a) When a public beach or public water-contact sports area fails to meet any of the standards as set forth in Section 7957 or 7958 above, the local health officer or the Department, after taking into consideration the causes therefor, may at his or its discretion close, post with warning signs, or otherwise restrict use of said public beach or public water-contact sports area, until such time as corrective action has been taken and the standards as set forth in 7957 and 7958 above are met.

Objective/Criterion Reference:

California Code of Regulations, Title 17, Section 7960

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Bacteriological monitoring samples were collected at Aliso Beach and South Laguna Beach in Orange County, California. Beaches included Emerald Bay, Crescent Bay, Laguna Main Beach, Hotel Laguna, Bluebird Canyon, Victoria Beach and Blue Lagoon. The posting covers 4.4 miles of beach shoreline.

Temporal Representation:

The beach closures covers the time frame of January 2000 -December 2006.

Environmental Conditions:

QAPP Information:

Samples were collected in compliance with County of Orange's Quality Assessment/Quality Control document (County of Orange, 2004).

QAPP Information Reference(s):

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

30301

Pollutant:

Indicator Bacteria

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

Not Recorded

Beneficial Use:

Water Contact Recreation

Number of Samples:

Number of Exceedances:

Data and Information Type:

PATHOGEN MONITORING

Data Used to Assess Water Quality:

Unspecified--This LOE is a placeholder to support a 303(d) listing decision made prior to 2006.

For the 2008 assessment , the 2006 listed coastline 'Pacific Ocean Shoreline, Laguna Beach HSA' was split into smaller segments and each represents an area near the sampling location of the data being assessed. This segment 'Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon' is one of the sampling locations. This LOE is a copy of the 2006 listing placeholder LOE for Pacific Ocean Shoreline, Laguna Beach HSA and is considered by the Regional Board to be applicable to this more specific segment formed from the split.

Data Reference:

Placeholder reference pre-2006 303(d)

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

Unspecified

Objective/Criterion Reference:

Placeholder reference pre-2006 303(d)

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Temporal Representation:

Environmental Conditions:

QAPP Information:

Unspecified

QAPP Information Reference(s):

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

77624

Pollutant:

Total Coliform

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Shellfish Harvesting

Number of Samples:

121

Number of Exceedances:

Data and Information Type:

PATHOGEN MONITORING

Data Used to Assess Water Quality:

Three of the 121 samples exceeded the objective of 70 mpn/100ml applied as a rolling 30 day geomean.

Data Reference:

Data for Region 9 Beach Watch.

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

The California Ocean Plan states that at all areas where shellfish may be harvested for human consumption, the median total coliform density shall not exceed 70 per 100 mL. Staff applied the objective as a rolling 30 day geometric mean consistent with other state bacteria objectives and with guidance from the National Shellfish Sanitation Program from which the objectives were taken.

Objective/Criterion Reference:

California Ocean Plan Water Quality Control Plan Ocean Waters of California 2009

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

The samples were collected at Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon.

Temporal Representation:

The samples were collected from January 2008 through August 2010.

Environmental Conditions:

QAPP Information:

The samples were collected for the beach watch program.

QAPP Information Reference(s):

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

29276

Pollutant:

Total Coliform

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Shellfish Harvesting

Number of Samples:

Number of Exceedances:

Data and Information Type:

PWS pathogen monitoring (ambient water)

Data Used to Assess Water Quality:

Beach monitoring data was collected by the County of Orange from January 1999 through December 2007. There were 939 individual samples collected with 51 samples correlated with storm events. Of the 51 samples, 26 exceeded single sample water quality objective. This information will not be used in determining a listing decision, but is of interest to the Regional Board and has been included here as additional anecdotal information.

Data Reference:

Ocean Bacteriological Data Evaluation for City of Laguna Beach, 1999 through 2006, Final Report

National Weather Service Forecast Office, San Diego, California, Chronological Regional Temperature and Precipitation Listings by Station

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

From the Ocean Plan, the median total coliform density shall not exceed 70 per 100 ml, and not more than 10 percent of the samples shall exceed 230 per 100 ml (SWRCB, 2005).

Objective/Criterion Reference:

Water Quality Control Plan Ocean Waters of California. California Ocean Plan 2005. Sacramento, CA: State Water Resources Control Board, California Environmental Protection Agency

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Samples were collected from Bluebird Canyon, station id S15, in the Laguna HSA. Lat/Long: 33.52963/-117.77359.

Temporal Representation:

Samples were collected weekly from January 1999 through December 2007; however, rainfall data is only available from March 2002 through December 2007.

Environmental Conditions:

QAPP Information:

Quality control for the bacteria analysis portion of this study was conducted in accordance with the County of Orange Quality Assessment/Quality Control document.

QAPP Information Reference(s):

County of Orange. Quality Assurance/Quality Control Manual, February 2004 Bacteria specific

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

29280

Pollutant:

Total Coliform

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Shellfish Harvesting

Number of Samples:

939

Number of Exceedances:

134

Data and Information Type:

PWS pathogen monitoring (ambient water)

Data Used to Assess Water Quality:

Beach monitoring data was collected by the County of Orange from January 1999 through December 2007. There were 939 individual samples collected with 134 exceeding the single sample water quality objective.

Data Reference:

Ocean Bacteriological Data Evaluation for City of Laguna Beach, 1999 through 2006, Final Report

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

From the Ocean Plan, the median total coliform density shall not exceed 70 per 100 ml, and not more than 10 percent of the samples shall exceed 230 per 100 ml.

Objective/Criterion Reference:

Water Quality Control Plan Ocean Waters of California. California Ocean Plan 2005. Sacramento, CA: State Water Resources Control Board, California Environmental Protection Agency

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Samples were collected from Bluebird Canyon, station id S15, in the Laguna HSA. Lat/Long: 33.52963/-117.77359.

Temporal Representation:

Samples were collected weekly from January 1999 through December 2007; however, rainfall data is only available from March 2002 through December 2007.

Environmental Conditions:

QAPP Information:

Quality control for the bacteria analysis portion of this study was conducted in accordance with the County of Orange Quality Assessment/Quality Control document.

QAPP Information Reference(s):

County of Orange. Quality Assurance/Quality Control Manual, February 2004 Bacteria specific

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

74903

Pollutant:

Total Coliform

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Shellfish Harvesting

Number of Samples:

142

Number of Exceedances:

Data and Information Type:

PATHOGEN MONITORING

Data Used to Assess Water Quality:

Water Board staff assessed Beach Watch data for Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon to determine beneficial use support and results are as follows: 5 of 142 samples exceed the criterion for Coliform, Total.

Data Reference:

Data for Region 9 Beach Watch.

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

The California Ocean Plan states that at all areas where shellfish may be harvested for human consumption, not more than 10 percent of the samples shall exceed 230 per 100 mL.

Objective/Criterion Reference:

California Ocean Plan Water Quality Control Plan Ocean Waters of California 2009

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Data for this line of evidence for Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon was collected at 1 monitoring site [ Bluebird Canyon]

Temporal Representation:

Data was collected over the time period 1/3/2008-8/30/2010.

Environmental Conditions:

Staff is not aware of any special conditions that might affect interpretation of the data.

QAPP Information:

The samples were collected for the Beach Watch program.

QAPP Information Reference(s):

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

29313

Pollutant:

Total Coliform

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

Number of Exceedances:

Data and Information Type:

PWS pathogen monitoring (ambient water)

Data Used to Assess Water Quality:

Beach monitoring data was collected by the County of Orange from January 1999 through December 2007. From March 2002 through December 2007, 51 samples correlated with storm events. Of the 51 samples, four exceeded the single sample water quality objective. This information will not be used in determining a listing decision, but is of interest to the Regional Board and has been included here as additional anecdotal information.

Data Reference:

Ocean Bacteriological Data Evaluation for City of Laguna Beach, 1999 through 2006, Final Report

National Weather Service Forecast Office, San Diego, California, Chronological Regional Temperature and Precipitation Listings by Station

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

Single Sample Maximum: Total coliform density shall not exceed 10,000 per 100ml;

Geomean: Total coliform density shall not exceed 1,000 per 100ml (SWRCB, 2005).

Objective/Criterion Reference:

Water Quality Control Plan Ocean Waters of California. California Ocean Plan 2005. Sacramento, CA: State Water Resources Control Board, California Environmental Protection Agency

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Samples were collected from Bluebird Canyon, station id S15, in the Laguna HSA. Lat/Long: 33.52963/-117.77359.

Temporal Representation:

Samples were collected weekly from January 1999 through December 2007; however, rainfall data is only available from March 2002 through December 2007.

Environmental Conditions:

QAPP Information:

Quality control for the bacteria analysis portion of this study was conducted in accordance with the County of Orange Quality Assessment/Quality Control document.

QAPP Information Reference(s):

County of Orange. Quality Assurance/Quality Control Manual, February 2004 Bacteria specific

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

74905

Pollutant:

Total Coliform

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

149

Number of Exceedances:

Data and Information Type:

Not Specified

Data Used to Assess Water Quality:

Zero of the 149 samples exceeded the total coliform objective. For this station, samples were collected from surfzone upcoast and surfzone downcoast. The results represent the higher of the two values.

Data Reference:

Data for Various Waterbodies in Region 8 and Region 9, 2006-2009.

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

The single sample total coliform concentration shall not exceed more than 10000/100ml. Water Quality Control Plan for Ocean Waters of California. Region 9 Basion Plan.

Objective/Criterion Reference:

Water Quality Control Plan for the San Diego Basin

Objective/Criterion Reference:

California Ocean Plan Water Quality Control Plan Ocean Waters of California 2009

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

The samples were collected from site BLUBRD, Coastal Stormdrain at Bluebird Canyon Drive (surfzone upcoast and downcoast).

Temporal Representation:

The samples were collected once a week from July 2006 to June 2009.

Environmental Conditions:

QAPP Information:

The samples were collected and analyzed under the Quality Assurance Management Plan for The Orange County Stormwater Program. Coastal Stormdrain Outfall Monitoring Program.

QAPP Information Reference(s):

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

29311

Pollutant:

Total Coliform

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

939

Number of Exceedances:

Data and Information Type:

PWS pathogen monitoring (ambient water)

Data Used to Assess Water Quality:

Beach monitoring data was collected by the County of Orange from January 1999 through December 2007. There were 939 individual samples with three exceeding the single sample water quality objective.

Data Reference:

Ocean Bacteriological Data Evaluation for City of Laguna Beach, 1999 through 2006, Final Report

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

Single Sample Maximum: Total coliform density shall not exceed 10,000 per 100ml;

Geomean: Total coliform density shall not exceed 1,000 per 100ml (SWRCB, 2005).

Objective/Criterion Reference:

Water Quality Control Plan Ocean Waters of California. California Ocean Plan 2005. Sacramento, CA: State Water Resources Control Board, California Environmental Protection Agency

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Samples were collected from Bluebird Canyon, station id S15, in the Laguna HSA. Lat/Long: 33.52963/-117.77359.

Temporal Representation:

Samples were collected weekly from January 1999 through December 2007; however, rainfall data is only available from March 2002 through December 2007.

Environmental Conditions:

QAPP Information:

Quality control for the bacteria analysis portion of this study was conducted in accordance with the County of Orange Quality Assessment/Quality Control document.

QAPP Information Reference(s):

County of Orange. Quality Assurance/Quality Control Manual, February 2004 Bacteria specific

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

74906

Pollutant:

Total Coliform

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

121

Number of Exceedances:

Data and Information Type:

Not Specified

Data Used to Assess Water Quality:

Zero of the 121 geomeans exceeded the objective.

Data Reference:

Data for Region 9 Beach Watch.

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

The geometric mean standard for total coliform states that the coliform density shall not exceed 1000 per 100 mL. Water Quality Control Plan for Ocean Waters 2009.

Objective/Criterion Reference:

California Ocean Plan Water Quality Control Plan Ocean Waters of California 2009

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Samples were collected at the Bluebird Canyon site.

Temporal Representation:

Samples were collected from January 2008 to August 2010.

Environmental Conditions:

QAPP Information:

The samples were collected for the Beach Watch program.

QAPP Information Reference(s):

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

29312

Pollutant:

Total Coliform

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

108

Number of Exceedances:

Data and Information Type:

PWS pathogen monitoring (ambient water)

Data Used to Assess Water Quality:

Beach monitoring data was collected by the County of Orange from January 1999 through December 2007. There were 939 individual samples with 108 monthly geomeans calculated. Of the 108 geomeans, none exceeded the geomean water quality objective.

Data Reference:

Ocean Bacteriological Data Evaluation for City of Laguna Beach, 1999 through 2006, Final Report

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

Single Sample Maximum: Total coliform density shall not exceed 10,000 per 100ml;

Geomean: Total coliform density shall not exceed 1,000 per 100ml (SWRCB, 2005).

Objective/Criterion Reference:

Water Quality Control Plan Ocean Waters of California. California Ocean Plan 2005. Sacramento, CA: State Water Resources Control Board, California Environmental Protection Agency

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Samples were collected from Bluebird Canyon, station id S15, in the Laguna HSA. Lat/Long: 33.52963/-117.77359.

Temporal Representation:

Samples were collected weekly from January 1999 through December 2007; however, rainfall data is only available from March 2002 through December 2007.

Environmental Conditions:

QAPP Information:

Quality control for the bacteria analysis portion of this study was conducted in accordance with the County of Orange Quality Assessment/Quality Control document.

QAPP Information Reference(s):

County of Orange. Quality Assurance/Quality Control Manual, February 2004 Bacteria specific

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

74904

Pollutant:

Total Coliform

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

138

Number of Exceedances:

Data and Information Type:

Not Specified

Data Used to Assess Water Quality:

None of the 138 samples exceeded the total coliform objective. For these stations, samples were collected from surfzone upcoast and surfzone downcoast. The higher of the two values was used for the geomean calculation.

Data Reference:

Data for Various Waterbodies in Region 8 and Region 9, 2006-2009.

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

The geometric mean for total coliform shall not exceed more than 1000/100ml. Water Quality Control Plan for the San Diego Basin. Water Quality Control Plan for Ocean Waters.

Objective/Criterion Reference:

Water Quality Control Plan for the San Diego Basin

Objective/Criterion Reference:

California Ocean Plan Water Quality Control Plan Ocean Waters of California 2009

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

The samples were collected from Coastal Stormdrain at Bluebird Canyon Drive (surfzone upcoast and surfzone downcoast).

Temporal Representation:

The samples were collected once a week from July 2006 to 2009.

Environmental Conditions:

QAPP Information:

The samples were collected and analyzed under the Quality Assurance Management Plan for The Orange County Stormwater Program. Coastal Stormdrain Outfall Monitoring Program.

QAPP Information Reference(s):

Line of Evidence (LOE) for Decision ID 77516, Indicator Bacteria	Region 9
Pacific Ocean Shoreline, Laguna Beach HSA, at Bluebird Canyon

LOE ID:

31079

Pollutant:

Total Coliform

LOE Subgroup:

Pollutant-Water

Matrix:

Water

Fraction:

None

Beneficial Use:

Water Contact Recreation

Number of Samples:

Number of Exceedances:

Data and Information Type:

PWS pathogen monitoring (ambient water)

Data Used to Assess Water Quality:

Data Reference:

Ocean Bacteriological Data Evaluation for City of Laguna Beach, 1999 through 2006, Final Report

SWAMP Data:

Non-SWAMP

Water Quality Objective/Criterion:

Geomean: Total coliform density shall not exceed 1,000 per 100ml within any 30 day period (SWRCB, 2005).

Objective/Criterion Reference:

Water Quality Control Plan Ocean Waters of California. California Ocean Plan 2005. Sacramento, CA: State Water Resources Control Board, California Environmental Protection Agency

Evaluation Guideline:

Guideline Reference:

Spatial Representation:

Samples were collected from Bluebird Canyon, station id S15, in the Laguna HSA. Lat/Long: 33.52963/-117.77359.

Temporal Representation:

Environmental Conditions:

QAPP Information:

Quality control for the bacteria analysis portion of this study was conducted in accordance with the County of Orange Quality Assessment/Quality Control document.

QAPP Information Reference(s):

County of Orange. Quality Assurance/Quality Control Manual, February 2004 Bacteria specific